Five simple, accurate, sensitive and economical UV spectrophotometric methods (A-E) have been developed and\r\nsubsequently validated for the determination of avitriptan in bulk and pharmaceutical formulations. The methods were based\r\non the formulation of colored complex of avitriptan with different reagents. The absorbance of the formed color complex is\r\nmeasured at the wavelength of maximum absorbance of the complex against the reagent blank. Method A is based on the\r\nformation of oxidative coupling reaction involving the use of iron (III) ââ?¬â?? MBTH (3-methyl-2-benzo thiozolinone hydrozone\r\nhydrochloride). The resulting green colored chromogen complex absorbs at max = 500 nm. Method B is based on the reaction of\r\navitriptan with ferric chloride and K3[Fe(CN)6] to form a green colored species having absorption maxima at max =760 nm.\r\nMethod C is based on the reaction of avitriptan with FeCl3 and 1,10-phenanthroline to form a blood red colored chromogen,\r\nexhibiting absorption maxima at max = 610 nm. Method D is based on the formation of oxidative coupling of drug with brucine\r\nin the presence of sodium meta periodate a purple red colored species is found and exhibit absorption maxima at max = 610 nm.\r\nMethod E is based on the formation of complex with acidic dye WFB BL having absorption maxima at max = 620 nm. All these\r\nmethods have different linearity ranges. Statistical analysis proves that the proposed methods are reproducible and selective for\r\nthe estimation of avitriptan in bulk drug and in its tablet dosage form.
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